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Ashton Embry's extensive research into the causes of Multiple Sclerosis and the Vitamin D connection have led him to a larger interest in Vitamin D and its effects on all aspects of human health.
This answer choice is grammatically incorrect: the singular subject research does not agree with the plural verb have.
What helps us identify this question as a Subject Verb Agreement question and identify the mistake is the following Stop Sign:
A long complex subject / a long distance between the subject and the verb
While this answer choice corrects the original mistake, it changes the original meaning by replacing the plural noun causes with the singular cause.
While this answer choice corrects the original Subject Verb Agreement mistake, it replaces the single word and with the three word phrase as well as. This makes this answer choice stylistically flawed (redundant). The use of the word including at the end of the underlined section, is similarly awkward.
While this answer choice corrects the original Subject Verb Agreement mistake, it changes the original meaning by replacing the plural noun causes with the singular cause. Also, it further changes the original meaning by omitting the word larger before the word interest.
This answer choice corrects the original Subject Verb Agreement mistake, by changing the plural verb have to the singular verb has, to match the singular subject research.